LOCUS       40924_4689        6188 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pAQ-EX1 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6188)
  AUTHORS   Miyasaka H, Nakano H, Akiyama H, Kanai S, Hirano M.
  TITLE     Production of PHA(poly hydroxyalkanoate) by the genetically 
            engineered marine cyanobacterium
  JOURNAL   Stud. Surf. Sci. Catal. 114, 237-242 (1998)
REFERENCE   2  (bases 1 to 6188)
  AUTHORS   Onizuka T, Miyasaka H, Akiyama H, Kanai S, Hirano M.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2001) Takuo Onizuka, Toray Research Center, Inc., 
            Biological Science Department; 1111 Tebiro, Kamakura, Kanagawa 
            248-8555, Japan (E-mail:Takuo_Onizuka@trc.toray.co.jp, 
            Tel:81-467-32-9962, Fax:81-467-32-0414)
REFERENCE   3  (bases 1 to 6188)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6188)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stud. Surf.
            Sci. Catal. 114, 237-242 (1998)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-SEP-2001) Takuo Onizuka, Toray Research Center, Inc., Biological
            Science Department"; volume: " 1111 Tebiro, Kamakura, Kanagawa 
            248-8555, Japan (E-mail:Takuo_Onizuka@trc.toray.co.jp, 
            Tel:81-467-32-9962, Fax"; pages: "81-467-32-0414"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6188
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      405..708
                     /note="promoter for Synechococcus sp. PCC 7002 Rubisco gene
                     (D13971)"
                     /regulatory_class="promoter"
     misc_feature    717
                     /label=PstI cloning site
                     /note="PstI cloning site"
     misc_feature    723
                     /label=BamHI cloning site
                     /note="BamHI cloning site"
     misc_feature    727
                     /label=SmaI cloning site
                     /note="SmaI cloning site"
     misc_feature    731
                     /label=KpnI cloning site
                     /note="KpnI cloning site"
     CDS             complement(935..3766)
                     /codon_start=1
                     /gene="orf943"
                     /product="ORF943"
                     /function="essential for transformation of cyanobacteria"
                     /label=orf943
                     /protein_id="BAB68386.1"
                     /translation="MTPYEYLLYSDEPALRRNDGRLRDTWLKRYAFVEHGGWWCSGIDI
                     KTGKDSLWGCFKGDRPRKDREDKKPIKYEHPPRVATEIFTLKVDRGTWRKIAKRHKVEL
                     PETDQGFWEWVLAHPELPIIITEGAKKAGALLTAGYCAIGLPGIYNGYRTPKNDHGEPM
                     RQLRHLIPELDLLAKNNRAIAFCFDQDKKPKTIKAVNGAIQTTGALLEKAGAKVSVITW
                     HQDAKGVDDLIVEHGAKALHNRYKHRKPLAVWEMDNLTDITTQVDLTVDQRYLDIDPRA
                     IPKDAQIIFIKSAKGTGKTEWLGKIVKLAQDDCARVLVLTHRIQLAKELARRLDIDHIS
                     ELDSSPTGGALGMAMCIDSLHPDSQAHFNFMEWHGAHIVLDEIEQVLGHALGSSTCTQD
                     RAKILETFYNLILYALRTGGKLYCSDADLSPISYELIKYILDGCEFKPFTILNTYKPCL
                     EQQRDLFFYEGNDPRDLLTNLRQAIENGEKTLVFTAAQKTASTYSTQNLESLFREKYPD
                     KRILRIDAESVAEPGHPAYGCIDSLNAILPLYDIVLCSPAVETGVSIDIKDHFDSVWGM
                     GSGVQTVNGFCQGLERLRDNVPRHVWIPKFSPHSNRIANGGYTAKAIARDQHRYAELTH
                     KLIGEHAAECSGLEDSLKPFLWAYCRYAALANRGFGSYREAILNKLLSEGYVQKDLSEI
                     DPALAKDYRDELKAVKDHNYLQERVAISKVENPDDRQYEKLKRQRAKSETERHQERHGK
                     LSRSYGLTVTPELVEKDDDGWYSQLQLEYYLTVGKAFCSARDRAKYDQLQHEGFVFKPD
                     INRRSLSPKIHLLELLNIHQFLKPGVTFTGASLEGFKENCLRYAKPIKWILGRTITDKM
                     SPLEIAQALLGKLDRKLEYKGRFGSRDNRQRVYEAIAPNDQREKVFAHWLQRDQAKLGA
                     VSNPCINRFIQEA"
     gene            complement(935..3766)
                     /gene="orf943"
                     /label=orf943
     primer_bind     complement(3967..3983)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3991..4007)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4015..4045)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4060..4081)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4369..4957)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5131..5988)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5989..6093)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt tctagaattc gagcgggatt
      421 ttatggcttt tttaggtatt tttgtaaggg taaaataggc ccatcaaaca gcattagaaa
      481 tgctaatcag cccaaaaaac aaaagcaatc tttttttgtt gctaaaagat aaaaataagt
      541 cgaggctgtg gtaacatatc ccacagatta aagaaagtca taagacttga atcttcagaa
      601 ttttaaaaag cagttttgcc aacgtaagat ttttgaagtt ttcgaccaac aataccgtta
      661 ctggtatttg tctgttaaag ataagcattt ttgctggagg aaaaccccat gctagactgc
      721 agggatcccg ggtaccatcg atgaattcga gctcatgccc ccggtagtgc cctcaccctc
      781 agtgaggctt atgatctggc acagtctagg ggctaccaga agagcaaagg cgctttccgc
      841 aagggcataa aacgtcccga taccgcagag caaatcttga gattgtttgg gattgccaag
      901 ggagagctgc acaacgagta tttcgatcac gggtctaagc ctcctgaata aatctattta
      961 tacaggggtt ggacacggcc cctaattttg cttggtcacg ctgtaaccaa tgagcaaaga
     1021 ccttttcgcg ctgatcgtta ggggcgatcg cctcatagac ccgctgacgg ttatcccgcg
     1081 atccaaagcg ccccttgtat tccaatttcc ggtcaagctt gcctaggagc gcctgagcaa
     1141 tttcgagcgg gctcattttg tcggtgatcg ttctgccaag aatccacttg atcggcttgg
     1201 cataccgcaa acaattttcc ttgaaccctt caaggctcgc cccggtgaat gtcacccctg
     1261 gttttaagaa ctgatggatg ttgagtagct ctaacaggtg aatctttggt gagagcgatc
     1321 gcctgttgat atccggctta aatacaaagc cctcatgttg gagctggtca tatttcgccc
     1381 ggtcgcgggc agagcagaat gctttcccaa cggttaagta gtattcgagc tggagctgag
     1441 agtaccaccc atcatcatct ttctcgacaa gctcaggggt cacagttaac ccataggagc
     1501 gagaaagttt cccgtggcgt tcttggtgcc gttccgtctc agatttcgcc cgctgacgct
     1561 tcagtttttc gtactggcga tcgtcaggat tttctacttt agaaatcgca accctttcct
     1621 gtagataatt atggtctttc accgctttta attcgtctcg ataatcctta gccaatgctg
     1681 gatcgatttc gctcaaatct ttctgtacat agccctcaga aagcagctta tttaaaatcg
     1741 cttcccggta actgccaaag ccacggttag caagcgccgc atagcgacaa taggcccaaa
     1801 ggaatggttt taaagaatct tctaacccac tgcattcagc ggcgtgctca ccgattaatt
     1861 tgtgggtgag ctctgcatag cggtgctggt cacgggcgat cgccttagcg gtgtagcccc
     1921 cattggcgat ccggttcgag tgtggggaaa atttcggtat ccaaacatgg cgagggacgt
     1981 tatcccgtaa ccgctctagc ccttggcaga aaccgttaac ggtctgaacc cctgaaccca
     2041 tgccccaaac agaatcaaaa tgatccttga tatcgatact caccccggtc tcgacagcgg
     2101 gcgagcagag cacaatatcg tacaggggca aaattgcatt cagcgagtca atacaaccat
     2161 aggcagggtg ccccggttca gcgaccgatt cagcatcgat tctcaagatt ctcttatcgg
     2221 gatatttctc cctaaagagt gattctaagt tctgcgtgct gtaggtcgat gcggtctttt
     2281 gggcagcggt aaagacaagt gttttctcac cgttctcaat cgcttgtctg agattggtta
     2341 acaggtctct agggtcattc ccttcataga aaaacaggtc tctttgttgc tctaaacagg
     2401 gcttataggt attcagaatg gtgaatggtt tgaactcaca accgtcaaga atgtacttga
     2461 ttagctcata ggagatggga gataaatcag catcagagca gtagagtttt ccgcctgtcc
     2521 ttagggcata aaggattagg ttgtagaacg tttcaaggat tttcgcccgg tcttgggtac
     2581 aggtcgagct acccaaagcg tgccctaaaa cttgctcgat ttcgtctagg acaatgtgag
     2641 cgccgtgcca ttccatgaaa ttaaaatgag cttggctatc gggatgtagg ctatcgatac
     2701 acatcgccat ccctagagcg cccccggtcg ggctactgtc gagctcgcta atgtgatcga
     2761 tatcgagacg gcgggcgagc tccttggcta attggatgcg gtgagtcaaa accagtacgc
     2821 gagcgcaatc atcttgggcg agcttaacga ttttccctaa ccattctgtt ttcccggtgc
     2881 ccttggcaga tttaatgaaa ataatctgag catccttggg gatagcacgc ggatcgatgt
     2941 cgagatagcg ctgatcgacc gttagatcga cttgcgtggt gatatcggtg agattatcca
     3001 tctcccagac tgctaagggc ttgcggtgct tgtagcggtt atggagtgct ttcgctccgt
     3061 gctcgacgat cagatcatca acacctttcg cgtcctggtg ccaggtgatc accgatactt
     3121 tcgccccggc cttctctagg agtgccccgg tagtttggat cgccccgttc actgccttga
     3181 tcgtcttggg tttcttgtct tggtcaaaac agaaggcgat cgcccggtta tttttcgcca
     3241 acaggtcaag ctctggaatg aggtgccgta gctgtcgcat tggctcgcca tggtcatttt
     3301 ttggcgttct gtagccgttg taaatccccg gtagaccgat ggcgcaataa ccagcggtta
     3361 ggagggcccc tgccttcttc gcgccctcag tgatgatgat cggtagctca ggatgggcta
     3421 gtacccattc ccagaagcct tgatcggttt ctggtagctc gactttgtgg cgcttggcaa
     3481 tcttgcgcca ggtgccccgg tctaccttga gagtgaaaat ttcggtggct acccgtggcg
     3541 ggtgctcata cttgatcggt ttcttatctt cgcgatcttt acgggggcga tcgcctttga
     3601 agcaacccca aagagaatct ttcccggtct taatatcgat gcctgagcac caccaaccgc
     3661 catgctcgac aaaggcatat cgcttaagcc aagtatcccg aagcctgcca tcattgcggc
     3721 gtaatgctgg ctcatcactg tagagcaggt attcataggg cgtcatccct gacaacgatc
     3781 taacatttaa ccgggcaatc tcaggggcga ccccggaacc ctctacccat tccctatagt
     3841 gagattctgc gataaaatga gaacggttct cactgacgct actcgctaaa tctgccccgg
     3901 tattactgct agatgtaaaa ttatctgata cgatcacagt gagttaagga gcttggcgta
     3961 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     4021 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     4081 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     4141 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     4201 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     4261 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     4321 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     4381 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     4441 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     4501 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     4561 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     4621 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     4681 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     4741 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     4801 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     4861 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     4921 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     4981 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     5041 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     5101 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     5161 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     5221 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     5281 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     5341 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     5401 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     5461 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     5521 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     5581 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     5641 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     5701 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     5761 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     5821 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     5881 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     5941 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     6001 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     6061 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     6121 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc
     6181 ctttcgtc
//
